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Journal: Structure (London, England : 1993)

Article Title: Structural Basis of CD160:HVEM Recognition

doi: 10.1016/j.str.2019.05.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6xHis lag antibody APC-conjugated Abeam Cat#ab72467; RRID: AB_1267596 Anti-6xHis tag antibody PE-conjugated Abeam Cat#ab72579: RRID: AB_1267597 Goat anti-human IgG FC (FITC) Abeam Cat#ab97224: RRID: AB_10680849 Bacterial and Virus Strains E.coli BL21(DE3) pLysS Promega N/A E.coli methionine auxotroph B834(DE3) Novagen N/A Chemicals, Peptides, and Recombinant Proteins PEI, MW 25000 Polysciences Inc. Cat#23966–1 Recombinant human HVEM Fc R&D systems Cat#356-HV/CF Recombinant human BTLA His-lag R&D systems Cat#9235-BT-050 Recombinant human CP160His-tag R&D systems Cat6177-CD-0S0 Critical Commercial Assays MCSG crystallization suite Anatrace MCSG-1 MCSG crystallization suite Anatrace MCSG-2 MCSG crystallization suits Anatrace MCSG-3 MCSG crystallization suite Anatrace MCSG-4 Deposited Data Crystal structure of gD:HVEM complex ( Carfi et al., 2001 ) PDB: 1JMA Crystal structure of BLA:HVEM complex ( Compaan et al., 2005 ) PDB:2AW2 Crystal structure oi TNF:TNFR2 complex ( Mukai et al., 2010 ) PDB:3ALQ Crystal structure of equine lentivirus recpetor 1 ( Qian et al. 2015 ) PDB:3WVT Crystal structure of HVEM ( Liu et al., 2015 ) PDB:4FHQ Crystal structure of Fab:LTα1β2:LTβR complex ( Sudhamsu et al., 2013 ) PDB:4MXW Crystal structure of Fab:CD40 complex ( Yu et al., 2018 ) PDB:6FAX Crystal structure of sc_CD160:HVEM This paper PDB:6NG3 Crystal structure of CD160 This paper PDB:6NG9 Crystal structure of SeMet V58M CD160 This paper PDB:6NGG Experimental Models: Cell Lima Drosophila S2 Thermo Fisher N/A HEK293 Thermo Fisher N/A Recombinant UNA Human CD160-pET3a This paper N/A Human BTLA-pET3a This paper N/A Human single chain CD16D-HVEM-pMT-Bip-V5-His This paper N/A Human CD160-pMT-Blp-V5-His This paper N/A Human CD160-pmCherry-N1 This paper N/A Human HVEM pGFP-N1 This paper N/A HSV1-gD-plRES-acGFP This paper N/A Software and Algorithms HHL2000 ( Otwinowski and Minor, 1997 ) http://www.hkl-xray.com/ SHELX ( Sheldrick, 2010 ) http://shelx.uni-goettingen.de/ CCP4 ( Winn et al., 2011 ) https://www.ccp4.ac.uk/ COOT ( Emsley et al., 2010 ) https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ ARP/wARP ( Winn et al., 2011 ) http://www.embt-hamburg.de/ARP/ REFMAC5 ( Winn et al., 2011 ) http://www.ccp4.ac.uk/html/refmac5.html Pymol Molecular Graphics System, Schrodinger, LLC https://pyimol.org/2/ CLUSTALW ( Thompson et al., 1994 ) https://www.genome.jp/tools-bin/clustalw ESRript 3.0 ( Robert and Gouet, 2014 ) https://espript.ibcp.fr/ESPript/ESPript/ PDBePISA ( Krissinel and Henrick, 2007 ) http://wwiw.ebi.ac.uk/pdbe/pisa/ Prism 5 Graphpad https://www.graphpad.com/scientific-software/prism/ Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Recombinant, Crystallization Assay, Software